Plasmid
GLR1pr

Part:BBa_K3600004:Design

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-14)


GLR1 promoter part plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 778
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 778
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 624
    Illegal BsaI.rc site found at 1145


Design Notes

The sequence of 500bp preceding the ORF of GLR1 was retrieved from the yeast genome database. No modifications were applied.

Source

Promoter of the glutathione reductase (YPL091W) from the genome of S. Cerevisiae.

References